Part I: Molecular and Cellular analysis and manipulation 1. Development of a Multi-Target Protein Biomarker Assay for Circulating Tumor Cells Diya Li, Ceming Wang, Yingjia Ni, Yaoping Liu, Wei Wang, Siyuan Zhang, Hsueh-Chia Chang, and Satyajyoti Senapati 2. Method to Isolate Dormant Cancer Cells from Heterogeneous Populations Julian A. Preciado, and Alptekin Aksan 3. Label-Free Morphological Phenotyping of in vitro 3D Micro Tumors Zoe Moscato, Devina Jaiswal, Krishna Dixit, Cooper J. Langanis, Kevin P. Claffey, and Kazunori Hoshino 4. High-throughput microenvironment microarray (MEMA) (file #high-resolution imaging Tiina A Jokela, Michael E Todhunter, and Mark A LaBarge 5. Real-Time Analysis of AKT Signaling Activities at Single-Cell Resolution Using Cyclic Peptide-Based Probes Fei Ji, Siwen Wang, Shiqun Shao, Priyanka Sarkar, and Min Xue 6. Microfluidic device technologies for digestion, disaggregation, and filtration of tissue samples for single cell applications Jeremy A. Lombardo, and Jered B. Haun 7. Microdissection methods utilizing single cell subtype analysis and the impact on precision medicine Donald J. Johann, Jr., Sarah Laun, Owen Stephens, Robert Weigman, , Ikjae Shin, , Adam Roberge, Meeiyueh Liu, Valerie Greisman, Mathew Steliga, Jason Muesse, , Erich Peterson, , Michael R. Emmert-Buck and Michael A. Tangrea, 8. Functionalized lineage tracing for the study and manipulation of heterogeneous cell populations Andrea Gardner, Daylin Morgan, Aziz Al'Khafaji, and Amy Brock 9. Fluorescence lifetime imaging probes for cell-based measurements of enzyme activity Sampreeti Jena, and Laurie L. Parker 10. Assessment of intracellular GTP levels using genetically encoded fluorescent sensors' Anna Bianchi-Smiraglia, and Mikhail. Nikiforov 11. Node-Pore Sensing for characterizing cells and extracellular vesicles Thomas Carey, Brian Li, and Lydia L. Sohn 12. Affinity-Based Enrichment of Extracellular Vesicles with Lipid Nanoprobes Yuan Wan, Mackenzie Maurer, and Si-Yang Zheng 13. Droplet magnetofluidic assay platform for quantitative methylation-specific PCR Alejandro Stark, Alexander Trick, Thomas R Pisanic II, and Tza-Huei Wang 14. Droplette: A Platform Technology to Directly Deliver Nucleic Acid Therapeutics and Other Molecules into Cells and Deep into Tissue Without Transfection Reagents Bao Lin Quek, Rathi L Srinivas, and Madhavi P Gavini 15. Molecular imaging of HER2 in patient tissues with touch prep-quantitative single molecule localization microscopy Devin L. Wakefield, Steven J. Tobin, Daniel Schmolze, and Tijana Jovanovic-Talisman 16. Microchip free-flow electrophoresis for bioanalysis, sensing and purification William E. Arter, Kadi L. Saar, Therese W. Herling, and Tuomas P. J. Knowles 17. Green Chemistry Preservation and Extraction of Biospecimens for Multi-omic Analyses Andrey P. Tikunov, Jeremiah D. Tipton, Timothy J. Garrett, Sachi V. Shinde, Hong Jin Kim, David A. Gerber, Laura E. Herring, Lee M. Graves, and Jeffrey M. Macdonald 18. TdT-UTP DSB End Labeling (TUDEL), for Specific, Direct in situ Labeling of DNA Double Strand Breaks Julian Lutze, Sara E Warrington, and Stephen J. Kron 19. Ligand-directed GPCR Antibody Discovery Qi Zhao, Amanda Chapman, Yan Huang, Mary Ferguson, Shannon McBride, Meghan Kelly, Michael Weiner, and Xiaofeng Li 20. Self-Induced Back-Action Actuated Nanopore Electrophoresis (Sane) (F...