Potential of model-based design of experiments approaches for bioprocess scale-down.- Digital Twins and Their Role in Model-Assisted Design of Experiments.- Digital twins for bioprocess control strategy development and realization.- The Kalman filter for the supervision of cultivation processes.-The challenge of implementing digital twins in operating value chains.- Digital twins in the (bio)pharma industry.- Numerical methods for the design and description of in vitro expansion processes of human mesenchymal stem cells.- Euler-Lagrangian Simulations: A Proper Tool for Predicting Cellular Performance in Industrial Scale Bioreactors.