Polysaccharide Degrading Biocatalysts provides a thorough grounding in these biocatalytic processes and their growing role in the depolymerization of polysaccharides, thus empowering researchers to discover and develop new enzyme-based approaches across pharmaceuticals, fuels and food engineering. Here, over a dozen leading experts offer a close examination of structural polysaccharides, genetic modification of polysaccharides, polysaccharide degradation routes, pretreatments for enzymatic hydrolysis, hemicellulose degrading enzymes, biomass valorization processes, oligosaccharide production, and enzyme immobilization for hydrolysis of polysaccharides, among other topics and related research protocols. Final sections consider perspectives and challenges in an evolving carbohydrate-based economy.