1. Introduction to in silico Modeling to Study ROS Dynamics Jana Schleicher 2. Assessment of ROS Production in Mitochondria of Live Cells Plamena R. Angelova, Albena T. Dinkova-Kostova, and Andrey Y. Abramov 3. Measurement of Superoxide Production in Acute Hypoxia by Fixed-Cell Microscopy Pablo Hernansanz-Agustíz, Carmen Choya-Foces, and Antonio Martínez-Ruiz 4. A Photodynamic Tool to Promote a Sustained, ROS-Dependent Growth of Human Hair Follicles in ex vivo Culture María I. Calvo-Sánchez, Sandra Fernández-Martos, and Jesús Espada 5. Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thaliana Liangsheng Wang and Tatjana Kleine 6. Characterization of oxidative lipidomics and autophagy induction in Chlamydomonas reinhardtii under abiotic stress Cristina Ortega-Villasante, Ángel Barón-Sola, Margarita Toledo-Basantes, Flor Martínez, and Luis E. Hernández 7. Fluorescence Detection of Increased Reactive Oxygen Species Levels in Saccharomyces cerevisiae at the Diauxic Shift Abhishek Sinha and Elah Pick 8. Flow Cytometry and Confocal Microscopy for ROS Evaluation in Fish and Human Spermatozoa Vanesa Robles, Marta F. Riesco, Juan M. Martínez-Vázquez, and David G. Valcarce 9. Measurement of Reactive Oxygen Species In Semen Samples Using Chemiluminescence Tânia R. Dias 10. Methods to Unravel Pathways of Reactive Oxygen Species in the Photodynamic Inactivation of Bacteria Natalia S. Gsponer and Edgardo N. Durantini 11. Revealing ROS Production by Antibiotics and Photosensitizers in Biofilms. A Fluorescence Microscopy Approach Sol R. Martínez and Andrés M. Durantini 12. EPR Spectroscopy as a Method for ROS Quantification in Skin Silke B. Lohan, Daniela Ivanov, Nadine Schüle, Bastian Berger, Stephanie Albrecht, and Martina C. Meinke 13. Detection of Superoxide Radical in Adherent Living Cells by Electron Paramagnetic Resonance (EPR) Spectroscopy Using Cyclic Nitrones Kahina Abbas, Nikola Babic, and Fabienne Peyrot 14. Singlet Oxygen Quantum Yield Determination Using Chemical Acceptors Roger Bresolí-Obach, Joaquim Torra, Renzo P. Zanocco, Antonio L. Zanocco, and Santi Nonell 15. A General Method to Establish the Relative Efficiency of Different Sonosensitizers to Generate ROS for SDT Viviana Grosso, Alejandro Senz, and Arnaldo T. Soltermann 16. Lipid Peroxidation Assay using BODIPY-phenylbutadiene Probes: A Methodological Overview Juan C. Stockert 17. Use of Fluorescent Probes to Detect ROS in Photodynamic Therapy Sulbha K Sharma and Michael R Hamblin