1. Cell Cycle Control: A System of Interlinking Oscillators Randy Y.C. Poon 2. Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity Matthew P. Swaffer 3. Real Time Monitoring of APC/C-Mediated Substrate Degradation Using Xenopus laevis Egg Extracts Julia Kamenz, Renping Qiao, Qiong Yang, and James E. Ferrell, Jr. 4. Fluorescent Peptide Biosensors for Probing CDK Kinase Activity in Cell Extracts Morgan Pellerano and May C. Morris 5. Phosphatase and Kinase Substrate Specificity Profiling with Pooled Synthetic Peptides and Mass Spectrometry Andrew G. DeMarco, Pete E. Pascuzzi, W. Andy Tao, and Mark C. Hall 6. Whole-Mount Immunostaining for the Identification of Histone Modifications in the S-Phase Nuclei of Arabidopsis Roots Hirotomo Takatsuka and Masaaki Umeda 7. Construction of a Full-Length 3'UTR Reporter System for Identification of Cell-Cycle Regulating MicroRNAs Dominika Kazmierczak and Per Hydbring 8. Purification of Cyclin-Dependent Kinase Fusion Complexes for In Vitro Analysis Mardo Kõivomägi 9. Optimizing Cell Synchronization Using Nocodazole or Double Thymidine Block Arif A. Surani, Sergio L. Colombo, George Barlow, Gemma A. Foulds, and Cristina Montiel-Duarte 10. Highly Synchronous Mitotic Progression in Schizosaccharomyces pombe Upon Relief of Transient Cdc2-asM17 Inhibition Pawan Singh, Lenka Halova, and Iain Michael Hagan 11. Elucidating Human Mitosis Using an Anaphase-Like Cell-Free System Danit Wasserman, Sapir Nachum, Meirav Noach-Hirsh, Naomi Auerbach, Evelin Sheinberger-Chorni, Taylor P Enrico, Roxane Lahmi, Michael J. Emanuele, and Amit Tzur 12. EDU (5-Ethynyl-2'-Deoxyuridine)-Coupled-Fluorescence-Intensity Analysis: Determining Absolute Parameters of the Cell Cycle João A. Ferreira, Marco Neves, Miguel Alpalhão, Pedro Pereira, Daniela Cunha, Fernando Ferreira, René Santus, Ana E. Sousa, and Paulo L. Filipe 13. High-Resolution Analysis of Centrosome Behavior During Mitosis Vanessa Nunes, Margarida Dantas, Joana T. Lima, and Jorge G. Ferreira 14. Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells Jonathan M. Geisinger and Tim Stearns 15. Use of Mitotic Protein Kinase Inhibitors and Phospho-Specific Antibodies to Monitor Protein Phosphorylation During the Cell Cycle Isha Nasa, Greg B. Moorhead, and Arminja N. Kettenbach 16. Visualization of Radiation-Induced Cell Cyc