Chapter 1_Absorption Spectroscopy. -Chapter 2_Mapping DNA Conformations by Circular Dichroism Spectroscopy. -Chapter 3_Circular Dichorism Spectroscopy: Exploring the Protein secondary and tertiary structure. -Chapter 4_Protein/Ligand interaction using Steady-state fluorescence spectroscopy. -Chapter 5_Unraveling the biomolecular kinetics and dynamics using Fluorescence Lifetime. -Chapter 6_Fluorescence Anisotropy: Probing Rotational Dynamics of Biomolecules.- Chapter 7_From Ensemble FRET to Single molecule Imaging: Monitoring individual cellular machinery in action. -Chapter 8_Exploring supramolecular chemistry using fluorescence as a basic tool. -Chapter 9_Fluorescence Correlation Spectroscopy : A novel single molecule tool for Biomolecular applications. -Chapter 10_Fluorescence microscopy for Biomolecules: Principle and Applications.-Chapter 11_Biomolecular alteration in the disease associated protein repertoire revealed by FTIR spectroscopy. -Chapter 12_Advancement in Raman Spectroscopy towards Biomolecular structure and Dynamics.