Part I: Virus and Virus Protein Production 1. Virus Propagation and Plaque Assay for Dengue Virus Pedro Henrique Carneiro, Lucas Mendes-Monteiro, and Ronaldo Mohana-Borges 2. Transmission Electron Microscopy Studies of Dengue Viruses Gabriela Cardoso Caldas, Ortrud Monika Barth, and Debora Ferreira Barreto-Vieira 3. Production of Properly Folded NS1 Protein in Bacterial Cells Diego Allonso 4. Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System Diego R. Coelho and Pedro Henrique Carneiro 5. Purification of Dengue and Zika Virus Non-Structural Protein 5 for Crystallization and Screening of Antivirals Aicha Gharbi-Ayachi, Abbas El Sahili, and Julien Lescar 6. Obtention of Dengue Virus Membrane Proteins and Role for Virus Assembly Szu-Chia Hsieh, Wen-Yang Tsai, and Wei-Kung Wang 7. Construction of Genomic and Sub-Genomic Dengue Infectious Replicons Thu M. Cao and Richard J. Kuhn Part II: Interaction between Dengue Virus and Cellular Proteins 8. Dengue Virus Capsid-Protein Dynamics in Live Infected Cells Studied by Pair Correlation Analysis Manuela V. Gabriel, Ignacio Sallaberry, Guadalupe S. Costa Navarro, Enrico Gratton, Andrea V. Gamarnik, and Laura C. Estrada 9. Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions Jonas Nascimento Conde 10. Isolation and Identification of Dengue Virus Interactome with Human Plasma Proteins by Affinity Purification-Mass Spectrometry Vivian Huerta and Yassel Ramos Part III: Immunopathogensis and Diagnosis 11. Molecular Diagnosis Of Dengue Priscila C.G. Nunes, Monique R.Q. Lima, and Flávia B. dos Santos 12. Serological Diagnosis of Dengue Monique R.Q. Lima, Priscila C.G. Nunes, and Flávia B. dos Santos 13. Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies Jonas Nascimento Conde, Megan Mladinich, William Schutt, and Erich R. Mackow 14. Evaluation of DENV-Induced Endothelial Cell Permeability by Measurements of Transendothelial Electrical Resistance (TEER) and Extravasation of Proteins and Virus Lana Monteiro Meuren, Sharton Vinicius Antunes Coelho, and Luciana Barros de Arruda 15. In Vitro Cytokine Production by Dengue-Infected Human Monocyte-Derived Dendritic Cells Allan Henrique Depieri Cataneo, Juliano Bordignon, Pryscilla Fanini Wowk, and Guilherme Ferreira Silveira