Part I: Structural Bioinformatics 1. The Protein Data Bank Archive Sameer Velankar, Stephen K. Burley, Genji Kurisu, Jeffery C. Hoch, and John L. Markley 2. Computational Methods for the Elucidation of Protein Structure and Interactions Nicholas S. Edmunds and Liam J. McGuffin 3. Methods for Molecular Modeling of Protein Complexes Tejashree Rajaram Kanitkar,, Neeladri Sen, Sanjana Nair, Neelesh Soni, Kaustubh Amritkar, Yogendra Ramtirtha, and M.S. Madhusudhan Part II: Protein Production 4. High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus Ester Behiels and Jonathan Elegheert 5. Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small-Scale: Application for Structural Studies Ganna O. Krasnoselska, Maud Dumoux, Nadisha Gamage, Harish Cheruvara, James Birch, Andrew Quigley, and Raymond J. Owens 6. Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells Maren Schubert, Manfred Nimtz, Federico Bertoglio, Stefan Schmelz, Peer Lukat, and Joop van den Heuvel 7. SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering Hannah Crocker, Barbara Gorda, Martin Pelosse, Deepak Balaji Thimiri Govinda Raj, and Imre Berger 8. Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification Sylvain Geny, Simon Pichard, Arnaud Poterszman, and Jean-Paul Concordet 9. Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems Marlitt Stech, Nathanaël Rakotoarinoro, Tamara Teichmann, Anne Zemella, Lena Thoring, and Stefan Kubick Part III: Structure Determination 10. Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins Yanzhang Luo, Shengqi Xiang, Alessandra Lucini Paioni, Agnes Adler, Peter Jan Hooikaas, Jijumon A.S., Carsten Janke3, Anna Akhmanova, and Marc Baldus 11. Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources Pierre Aller and Allen M. Orville 12. From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example Christoph A. Diebolder, Rebecca S. Dillard, and Ludovic Renault 13. Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods Carlos Oscar S. Sorzano, Amaya Jiménez-Moreno, David Maluenda, Erney Ramírez-Aportela, Marta Martínez, Ana Cuervo, Robert Melero, Jose Javier Conesa, Ruben Sánchez-García, David Strelak, Jiri Filipovic, Estrella Fernández