The book contains 17 chapters categorized into 3 sections. The first section presents the latest information on bioinformatics tools, artificial intelligence, machine learning, computational methods, protein interactions, peptide-based drug design and omics technologies. The following 2 sections include bioinformatics tools for the pharmaceutical sector and for the healthcare sector. Bioinformatics brings a new era in research with the aim to accelerate drug target and vaccine design development, improve validation approaches as well as facilitate in identifying side effects and predict drug resistance. As such, this will aid in more successful drug candidates from discovery to clinical trials to the market, and most importantly making it a more cost-effective process overall.